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About Us
Instruments
Services
Protocols
MS Resources
Acknowledgments
 
Please acknowledge use of MS Facility instruments and staff services in publications as appropriate. Include the phrase "We wish to thank the UCI Mass Spectrometry Facility..." to facilitate collection of publication metrics. Some examples of sample text for facility services is provided below.
 

Accurate Mass Measurement
"We wish to thank the UCI Mass Spectrometry Facility and <staff name(s)> for assistance with small molecule analyses and accurate mass measurements. Data were collected on a Waters LCT Premier by flow injection analysis (FIA) in methanol and data analyzed with MassLynx v. 4.1 software. In ESI+ mode, analyte m/z ions (M+H)+ or (M+Na)+ were validated to less than ± 5 ppm relative to the nearest sodiated polyethylene glycol (CAS: 25322-68-3, av. Mwt 400)) or sodiated methoxypolyethyleneglycol (CAS: 990-74-4, av. Mwt 350) calibrant peak lockmass. In ESI- mode, analyte m/z ions (M-H)- were validated relative to the nearest calibrant peak from the sodium formate cluster series (m/z 100-2000).”

Peptide/intact Protein Services
"We wish to thank the UCI Mass Spectrometry Facility and <staff name(s)> for assistance with collection and analyses of protein mass spectrometry data. Data were collected on a Waters Acquity UPLC Xevo G2-XS QTOF system (NIH supplemental funding support received by: J.S. Nowick (NIGMS GM097562), Vy Y. Duong (NIH GM105938) and O. Cinquin (NIGMS GM102635)). Data were analyzed with Waters MassLynx v.4.1 and <name of software package>."

Quantitative Omics
"We wish to thank the UCI Mass Spectrometry Facility and <staff name(s)> for assistance with method development and quantitative LC-MS/MS analysis of <analyte names/classes>. Data were collected on a Waters Acquity UPLC Quattro Premier and/or Waters TQ Absolute Premier triple quadrupole systems. Instrument funding support was provided by UCI’s Office of Research, MS Facility, Schools of Physical Sciences/Engineering/Biological Sciences/Medicine and Pharmacology & Pharmaceutical Sciences."

Imaging Mass Spectrometry
"We wish to thank the UCI Mass Spectrometry Facility and <staff name(s)> for assistance with Imaging Mass Spectrometry (IMS) analyses. Data were collected on a Shimadzu iMScope 9050 (AP-MALDI QTOF) instrument and analysed in Shimadzu's ImageReveal software."

"We wish to thank the UCI Mass Spectrometry Facility and <staff name(s)> for assistance with Imaging Mass Spectrometry (IMS) analyses. Data were collected on a Bruker ultrafleXtreme MALDI-TOF instrument and analysed in Bruker’s SCiLS Lab Pro software."

 

Grant Applications
 
Facility Description, Equipment & Other Resources
 
The Mass Spectrometry Facility (MSF) in the Department of Chemistry provides staff services and walk-up access to a variety of mass spectrometry instruments suitable for the analysis of small molecules, peptides/proteins and nucleic acids. The MSF provides services principally to faculty research groups in Chemistry and other UC departments/schools, but can also accept samples from outside academic/non-profit and commercial clients.
 

The MSF operates 20 instruments that are equipped with liquid chromatography (LC), gas chromatography (GC) or matrix assisted laser desorption (MALDI) input sources coupled to quadrupole or time-of-flight (TOF) MS systems. All instruments have extensive sets of pre-programmed methods available that are suitable for the majority of samples typically encountered; method customization can also be accommodated.
 
Location:
1002 Reines Hall, Department of Chemistry, UC Irvine

Staff
Felix Grün, PhD (Director)
Ben Katz (Proteomics Specialist)
Chris Dicksion (Graduate MS Fellow)
Zane Long (Graduate MS Fellow)

 

Major Equipment
 
The MS Facility is equipped with twenty instruments.  There are fourteen Atmospheric Pressure Ionization (API) LC instruments. Ten are time-of-flight (TOF) systems and seven of these (two Synapt G2, QTOF Premier, Xevo G2-XS QTOF, iMScope-9030 QTOF, Thermo LTQ XL and Shimadzu IT-TOF) have MS/MS, ion trap and/or ion mobility separation capability. The facility has two Waters LCT Premiers, one LCT classic and two single quadrupole (QDA) systems, as well as two triple quadrupole LCMS instruments (Waters Quatro Premier XE and TQ-Absolute). In addition three facility instruments are Matrix Assisted Laser Desorption Ionization Time-of-Flight (MALDI-TOF/TOF) mass spectrometers (AB Sciex 5800,  Bruker ultrafleXtreme and Shimadzu iMScope AP-MALDI QTOF). The Bruker and Shimadzu MALDI systems are dedicated to MALDI imaging mass spectrometry applications. There are five gas chromatograph-mass spectrometers (GC-MS): a GC-TOF (Waters GCT Premier) and four quadrupole systems (Thermo and Agilent) suitable for liquid, headspace, SPME and thermal desorption samples. The facility performs intact accurate mass determination of small molecules, peptides and proteins, characterization of post-translational modifications, top-down and bottom-up proteomics, quantitative targeted and untargeted metabolomics and mass spectrometry imaging (IMS). 
 
The facility is staffed by an experienced Ph.D. level Director (Dr. Felix Grun), proteomics specialist (Ben Katz) and two instrument operators (Graduate MS Fellows). Eighteen out of twenty instruments, including all high-end instruments such as the Synapt G2, Xevo G2 XS-QTOF, triple quadrupoles, GC MS systems and MALDI-TOF instruments are available for walk-up Open Access mode to researchers on a 24/7 basis. This allows for both rapid turn-around of samples and the opportunity for students to obtain hands on experience in mass spectrometry. 
 
Other Resources
 
User and instrument training is provided weekly (Wednesdays 9:00-11:00 AM). Contact the Director to register. 
 
The facility also provides access to additional equipment including several HPLC Systems for analytical and semi-prep reverse phase/normal phase separations, a vacuum freeze dryer and rotary evaporator.

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